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Immunoprecipitation (IP)-mass spectrometry (MS) and Co-IP To identify proteins differentially interacting with WT PRDX1 and PRDX1 Cys52Ser mutant, pcDNA3.1-3xFlag-PRDX1 and pcDNA3.1-3xFlag-PRDX1 Cys52Ser expression constructs were transfected in HEK293T cells for 48 hr
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And perhaps most importantly, the rate of treatment discontinuation, which reveals how tolerable each treatment is in the real world rather than in the controlled environment of separate trials
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