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Recombinant Protein Expression In order to choose the most efficient baculovirus for purification and to monitor what time the peak of protein expression is reached, perform a mini-infection assay as follows: Infect 2 x 10 7 of Sf9 infected cells cultured at 27 °C in Grace's media (complemented with 10% FBS and 1% P/S) with 133 μl (1/150) of baculovirus