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Frontiers | LRRK2 regulates ArfGAP1 membrane localization, activity and neuronal integrity via phosphorylation within its lipid-sensing ALPS2 motif
Our attempts to verify these phospho-sites in cells co-expressing ArfGAP1 with LRRK2 variants combined with mass spectrometry analysis of immunoprecipitated ArfGAP1 were unsuccessful, most likely due to the low abundance and/or compartment-specific localization of these LRRK2-specific phosphorylation sites for detection in whole cell extracts
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