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What Is a His-Tag? Principles, Purification & Applications - Biofargo
Application data: Figure 4 Lane M: Marker Lane 1 :Start material Lane 2: Flowthrough 1 Lane 3: Flowthrough 2 Lane 4: Elution (20 mM Imidazole) Lane 5: Elution (50 mM Imidazole) Lane 6: Elution (100 mM Imidazole) Lane 7: Elution (200 mM Imidazole) Lane 8: Elution (300 mM Imidazole) Lane 9: Elution (500 mM Imidazole) Packing: Ni-IDA Sepharose FF (Gravity Preloaded Column) (Cat: abs91007-G), 1 mL Sample: his6-tagged recombinant Streptococcus Protein G produced in E
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Hum Vaccine Immunother (2020) 16(12):2938–43
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