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В качестве подвижной фазы применяли 20 мМ раствор фосфата натрия, 140 мМ NaCl, pH 7.4.Size exclusion chromatography (SEC) was performed on an Amersham Bioscience AEKTAbasic system equipped with a Пример 1Example 1 Синтез реагента основной цепи 1g и 1hSynthesis of the main chain reagent 1g and 1h Реагент основной цепи 1g был получен из амино 4-разветвленного PEG5000 1а согласно следующей схеме:The main chain reagent 1g was obtained from amino 4-branched PEG5000 1a according to the following scheme: Для синтеза соединения 1b, 4-разветвленный-РЕ05000 тетраамин 1а (мол
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