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Conversely, the VPA_RAT group showed higher species evenness, with elevated Shannon index (Figure S3D) and Simpson index (Figure S3E), indicating more balanced microbial distribution

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At the same time, compared to HCs, the levels of a wide range of metabolites such as glutamine, asparagine, N-Formimino-glutamate, putrescine, serotonin, kynurenine, 2-Aminomuconate, Cys-Gly, 5-Aminolevulinate, and pyridoxine were reduced in the plasma of R group ( Figure 5C , Supplementary Figure 4A )

including the regulation of immune function and metabolism.