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For example, the formation of an isopeptide bond between the sidechains of lysine and glutamine is as follows: Gln−(C=O)NH 2 + Lys-NH 3 + → Gln−(C=O)NH−Lys + NH 4 + The ε-amino group of lysine can also react with the α-carboxyl group of any other amino acid as in the following reaction: Ile-(C=O)O − + Lys-NH 3 + → Ile-(C=O)NH-Lys + H 2 O Isopeptide bond formation is typically enzyme-catalyzed
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Rotenone (Santa Cruz #203242) (50 μM) was used as a positive control
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