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Cell culture GAS7, ΔFFL1 (aa 171–197 deletion, GAS7cb residue numbers), ΔFFL2 (aa 209–216 deletion) and all of the amino-acid substitution mutants (all described in terms of the GAS7cb amino-acid residue number) were cloned into the pEGFP-C1 vector, and EGFP was substituted with its brighter variant, Venus 61