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To examine whether PlexinB1 activates the GTPase activity of Ran directly, purified cytoplasmic PlexinB1 protein of WT sequence (cytoPlexinB1-WT) or a mutant form of PlexinB1 with a non-functional GAP domain [23] (cytoPlexinB1-RA) were used in direct GTPase activating proteins (GAP) activity assays according to the method of Webb et al
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