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Here, we show that MVI also contains one putative nucleolar localization signal (NoLS), situated within the MVI cargo-binding domain, overlapping the bipartite NLS and containing motifs involved in the interaction with MVI partners and phospholipids (Figure 1A) ( Localization of MVI to the nucleolar subdomains as well as its newly revealed interaction with proteins specific to these regions, UBF, fibrillarin, and B23 suggest the involvement of MVI in ribosome biogenesis taking place in this nuclear compartment