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PCR amplification was carried out with SeqAmp DNA Polymerase (TaKaRa Bio) using a gene-specific primer containing a 15-bp overlap sequence for cloning (5′-gattacgccaagcttGCTCCATCTTCAGTGAGCTCGTGTGCGC-3′) under touchdown PCR conditions (Program 1)
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