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Real-Time qPCR Total RNA was extracted from lung tissues using an RNAeasy™ Animal RNA Isolation Kit with a Spin Column (Beyotime Biotechnology, Shanghai, China) and reverse-transcribed to cDNA using a FastKing cDNA First Strand Synthesis Kit (Tiangen, Beijing, China)
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Another commenter generally supported integrating rural clinicians into value-based care models but recommended that CMS allow an opt-out option for rural clinicians, if needed, because of additional burden related to a lack of bandwidth or readiness to participate
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