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Cells were stained for 30 min on ice, washed twice in FACS buffer, fixed in 200 µl of 10% formalin 15 min on ice, washed and resuspended in 200 µl FACS buffer for acquisition and analyzed on a BDFACS Celesta
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Thus, understanding the metabolic basis of Doxycycline-resistance has ultimately helped us to develop a new synthetic lethal strategy, for more effectively targeting CSCs
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