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224 The decrease in GSH and GPX4 leads to increased tissue damage in animal models of ferroptosis and diabetes

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Following further optimization in the PC activation process, for 1 µM (62.5 µg/ml) PC, the optimal thrombin concentration was 85 nM or 3.12 µg/ml (8.5% molar ratio or 5% w/w ratio to PC), whereas higher thrombin concentrations did not result in higher APC generation (see Supplementary Fig