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Knockdown decreased expression of autophagy markers ( Becn1 [Beclin1], Atg7 [autophagy related 7], Atg12 [autophagy related 12], Atg14 [autophagy related 14], Map1lc3b [LC3B]) while increasing Sqstm1 (SQSTM1/p62), indicating impaired autophagic flux [49]
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