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Nonetheless, the universe of applications for the IMAC/His-tag system is widely used for protein purification and is extended to matrix-assisted refolding (Dashivets et al., 2009), detection and immobilization, protein microarrays (Kato et al., 2005, Wegner et al., 2003, Wilson and Nock, 2002), and to the development of other affinity tag technologies

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