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FSH further controls the production and activity of FAAH in Sertoli cells by activating the protein kinase A (PKA)/aromatase pathway [132]

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1-butyl-3-(butylthio)-1 H -pyrrole (0.47 mmol, 1 equiv.) was dissolved in 10 mL of acetonitrile and added to a reaction mixture of dimethyl acetylenedicarboxylate (1.5 equiv.) in acetonitrile (10 mL) and the reaction mixture was stirred for 16 h at RT